DILUTION OF PERMANONE 4-8 ® FOR THE BOTTLE BIOASSAY
DRAFT DOCUMENT FOR REVIEW
PHEREC Technical Memorandum # 18
Start with the label. According to the label:
1 gallon of PERMANONE 4-8 ® contains .289 lbs of permethrin
1 gallon = 3.785 liters
1 pound (lb.) = 453.6 grams
0.289 lbs X 453.6 g/lb = 131 grams a.i./gal.
131/3.785 = 34.6 grams/liter
1 liter of PERMANONE 4-8 ® contains 34.6 grams of permethrin.
1 milliliter of PERMANONE 4-8 ® contains .0346 grams of permethrin.
1 milliliter of PERMANONE 4-8 ® contains 34.6 milligrams of permethrin.
TAKE one (1) milliliter of PERMANONE 4-8 ® and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.] This is the STOCK solution.
The STOCK solution contains 0.346 milligrams/ml
TAKE 12.4 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. This is the TEST solution.
.346 milligrams/ml X 12.4 ml = 4.3 milligrams of permethrin
4.3/100 = 0.043 milligrams/ml
This is equivalent to 43 micrograms/ml
The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin
USE 1 ml of the TEST solution in each bottle of the bioassay according to the protocol by Petersen et al. available at http://pherec.com/bottleassay/procedure.html
For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email firstname.lastname@example.org
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