DILUTION OF PERMANONE 4-8 ^{®} FOR THE BOTTLE BIOASSAY

**DRAFT DOCUMENT FOR REVIEW**

PHEREC Technical Memorandum # 18

**Start with the label. According to the label:**

1 gallon of PERMANONE 4-8 ^{®} contains .289 lbs of permethrin

1 gallon = 3.785 liters

1 pound (lb.) = 453.6 grams

0.289 lbs X 453.6 g/lb = 131 grams a.i./gal.

131/3.785 = 34.6 grams/liter

1 liter of PERMANONE 4-8 ^{®} contains 34.6 grams of permethrin.

1 milliliter of PERMANONE 4-8^{ ®} contains .0346 grams of permethrin.

1 milliliter of PERMANONE 4-8 ^{®} contains 34.6 milligrams of
permethrin.

TAKE one (1) milliliter of PERMANONE 4-8 ^{®} and, using a volumetric
flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. [A.C.S.
acetone is the grade of acetone approved by the American Chemical Society.]
This is the STOCK solution.

The STOCK solution contains 0.346 milligrams/ml

TAKE 12.4 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. This is the TEST solution.

.346 milligrams/ml X 12.4 ml = 4.3 milligrams of permethrin

4.3/100 = 0.043 milligrams/ml

This is equivalent to 43 micrograms/ml

The TEST solution contains 0.043 milligrams/ml permethrin

The TEST solution contains 43 micrograms/ml permethrin

USE 1 ml of the TEST solution in each bottle of the bioassay according to the
protocol by Petersen __et__ __al__. available at http://pherec.com/bottleassay/procedure.html

For more information, CONTACT Dr. Jack Petersen

at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Please check our Web site for updates on this protocol:

http://pherec.com

4/18/2011