PHEREC Technical Bulletin

DILUTION OF PERMANONE 4-8 FOR THE BOTTLE BIOASSAY

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 18

Start with the label.  According to the label:

1 gallon of PERMANONE 4-8 contains .289 lbs of permethrin

1 gallon = 3.785 liters
1 pound (lb.) = 453.6 grams

0.289 lbs X 453.6 g/lb = 131 grams a.i./gal.
131/3.785 = 34.6 grams/liter

1 liter of PERMANONE 4-8 contains 34.6 grams of permethrin.
1 milliliter of  PERMANONE 4-8 contains .0346 grams of permethrin.
1 milliliter of  PERMANONE 4-8 contains 34.6 milligrams of permethrin.

TAKE one (1) milliliter of PERMANONE 4-8   and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.   [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.]  This is the STOCK solution.

The STOCK solution contains  0.346 milligrams/ml

TAKE 12.4 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.  This is the TEST solution.

.346 milligrams/ml X 12.4 ml = 4.3 milligrams of permethrin
4.3/100 = 0.043 milligrams/ml

This is equivalent to 43 micrograms/ml

The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin

USE 1 ml of the TEST solution in each bottle of the bioassay according to the protocol by Petersen et al. available at http://pherec.com/bottleassay/procedure.html

 

For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Please check our Web site for updates on this protocol:
http://pherec.com

4/18/2011