FORMULATION of SPINOSAD for LARVAL BIOASSAYS
Start with the LABEL. The LABEL is the LAW. NATULAR 2ECTM CONTAINS 20 % SPINOSAD.
Take 1 ml of NATULAR 2EC, dilute with 99 ml ACS acetone in a 100 ml volumetric flask. This is the STOCK solution.
Take 1 ml of the STOCK solution, dilute with 99 ml ACS acetone in a 100 ml volumetric flask. This is the TREATMENT solution.
Take 1 ml of the TREATMENT solution, add it to 249 ml larval water in a bioassay cup.
100 ppm X .20 = 20 ppm AI [spinosad] [NATULAR is 20 % AI]
20 ppm = 20,000 ppb
20,000 ppb X 1/250 = 80 ppb
80 ppb is the diagnostic concentration based on dose response bioassays performed at Rutgers University.
This technical memorandum was developed in collaboration with Scott Crans, Center for Vector Biology, Rutgers University, as part of a training workshop.
For the larval bioassay protocol, CLICK HERE.
CAUTION: This is a draft document! We are still beta checking this protocol. Please check back for updates. Contact Dr. Jack Petersen, firstname.lastname@example.org with any questions or comments.
This technical memorandum was developed based on larval bioassays funded by the Florida Department of Agriculture and Consumer Services [FDACS] CONTRACT # 015013 (2010).
Created on Thursday, January 13, 2011. Amended: Friday, January 14, 2011. Edited: March 8, 2012 Edited again on May 1, 2014 and on February 21, 2015.