Here is a draft of the amended DIBROM CONCENTRATE EC technical memorandum.

The active ingredient in DIBROM is Naled.

1. Start with the label.
2. 1 gallon of DIBROM contains 13.2 pounds of Naled.
3. Convert pounds per gallon to grams per milliliter.
4. 13.2 lbs/gallon X 453.6 grams / lb X 1 gallon / 3785 ml = 1.58 grams of Naled / ml of DIBROM.
5. Take 1 ml of DIBROM and put it in a 100 ml volumetric flask.
6. Fill to 100 ml with A.C.S. acetone. Swirl to mix.
7. This is the STOCK SOLUTION.
8. Prepare the TEST SOLUTION as follows:
9. Take 0.158 ml of the STOCK SOLUTION and put it in a 100 ml volumetric flask.
10. Fill to 100 ml with A.C.S. acetone. Swirl to mix.
11. This is the TEST SOLUTION.

Measure 1 ml of the TEST SOLUTION into each 250 ml Wheaton bottle for the bioassay.

This procedure will yield 25 micrograms of Naled in each Wheaton bottle for the bioassay.

Contact Dr. Jack Petersen for more information: drjack3@hotmail.com

edited 15 June 2012